The Plastic Surgery Research Laboratory consists of 1200 square feet of space in the Biomedical Science Tower. Included in the lab is one 4′ chemical fume hood, three 4′ biological safety hoods, 5 incubators, 3 refrigerators, 2 freezers, as well as microscopes, balances, and traditional glassware and supplies. Our laboratory has the capabilities to study protein expression via Western Blotting and RNA expression via Northern Blotting. The department of surgery contains several core facilities, including a sterilization facility, constant temperature and cold temperature rooms, DI/RO water purification system, and a labware washing facility, all located on our floor. The laboratory is equipped with small equipment suitable for all proposed cell biology and protein chemistry experiments. Additionally, we have access to the following (through our collaborators): Flow cytometry, Instron, GPC, FT-IR, NMR, Histology Facilities, SEM, goniometer, and XPS.

MAJOR EQUIPMENT:

Thermolyne Harvery SterileMax autoclave; Sorvall Legend RT centrifuge

Tecan SpectraFluorPlus platereader; Labconco Freeze Dryer

American Optical temperature controlled water bath shaker

Accumet AB15 pH meter; Genesys 10vis Spectrometer; Gemoro Ultrasonic bath

Barnstead Nanopure Diamond water system; 2 Thermolyne Locator 8+ cell freezers

Zeiss AxioVert 25 inverted fluorescent microscope with imaging system

Olympus CKX31 Inverted microscope

ISC BioExpress vertical systems (casting base and blotters)

BioRad C1000 Thermal Cycler; BioRad ChemiDoc XRS + Imaging System;

Mitsubishi P93D Thermal Printer

LabNet Spectrafuge 24D; LabNet Mini Incubator

Wooden Walking Track (for rat SFI studies)

Basler A602f High Speed firewire camera (for gait kinematics)

Leco IA32 Image Analysis System

Hitachi CCD KP-M1AN digitizing camera